PharmD, PhD (Toxicology)

1/2013 – 6/2017

Applying 3D cell culture models for long-term hepatic in vitro toxicology applications.
I developed 3D models for differentiated cells (#1, #2) and stem cells (3). This gave me a strong experimental experience in cell and tissue engineering, histology, and gene expression as well as in implementing functional assays for the evaluation of cells biotransformation capacity and toxicological response (#1, #2). I had also the opportunity to work with HPLC methodologies for drug metabolites quantification and for the thiolomic profile determination (4, 5). In parallel with my project, I participated in several other projects comprehending: the study of stem cell conditioned medium for wound healing and tissue regeneration (3); for the modulation of breast cancer migration and invasion; the study of nevirapine toxicity mechanisms using a dynamic 3D culture of rat primary hepatocytes (5); and the study of efavirenz short- and long-term neurotoxic mechanisms in a rat model (4).

Publications from doctoral thesis (% referes to my contribution to each project)

# 1. [...], M., Correia, J. C., Camões, S. P., Oliveira, N. G., Cruz, P., Cruz, H., Castro, M., Ruas, J. L., Santos, J. M., Miranda, J. P. (2017) The role of epigenetic modifiers in extended cultures of functional hepatocyte-like cells derived from human neonatal mesenchymal stem cells. Arch Toxicol. 91(6):2469-89. (70% - study design, experimental work, data analysis and manuscript writing)
# 2. [...], M.#, Freyer, N., Knöspel, F., Oliveira, N. G., Barcia, R., Cruz, P. E., Cruz, H., Castro, M., Santos, J. M., Zeilinger, K., Miranda, J. P. (2017) Self-assembled 3D spheroids and hollow-fibre bioreactors improve MSC-derived hepatocyte-like cell maturation in vitro. Arch Toxicol. 91(4):1815-32. (80% - study design, experimental work, data analysis and manuscript writing)
3. Santos, J. M., Camões, S. P., Filipe, E., [...], M., Barcia, R. N., Filipe, M., Teixeira, M., Simoes, S., Gaspar, M., Mosqueira, D., Nascimento, D. S., Pinto-Do, O. P., Cruz, P., Cruz, H., Castro, M., Miranda, J. P. (2015) Three-dimensional spheroid cell culture of umbilical cord tissue-derived mesenchymal stromal cells leads to enhanced paracrine induction of wound healing. Stem Cell Res Ther. 6:90. (30% - experimental work, data analysis and manuscript writing)

4. Grilo, N. M., Joao Correia, M., Miranda, J. P., [...], M., Serpa, J., Matilde Marques, M., Monteiro, E. C., Antunes, A. M. M., Diogo, L. N., Pereira, S. A. (2017) Unmasking efavirenz neurotoxicity: Time matters to the underlying mechanisms. Eur J Pharm Sci. 105:47-54. (20% - experimental work, data analysis and manuscript writing)
5. Pinheiro, P. F., Pereira, S. A., Harjivan, S. G., Martins, I. L., Marinho, A. T., [...], M., Jacob, C. C., Oliveira, N. G., Castro, M. F., Marques, M. M., Antunes, A. M., Miranda, J. P. (2017) Hepatocyte spheroids as a competent in vitro system for drug biotransformation studies: nevirapine as a bioactivation case study. Arch Toxicol. 91(3):1199-211. (15% - experimental work and data analysis)
6. Fernandes, A. S., Florido, A., Saraiva, N., Cerqueira, S., Ramalhete, S., [...], M., Cabral, M. F., Miranda, J. P., Castro, M., Costa, J., Oliveira, N. G. (2015) Role of the Copper(II) Complex Cu[15]pyN5 in Intracellular ROS and Breast Cancer Cell Motility and Invasion. Chem Biol Drug Des. 86(4):578-88. (15% - experimental work and data analysis)
7. Fernandes, A. S., Costa, J., Gaspar, J., Rueff, J., Cabral, M. F., [...], M., Castro, M., Oliveira, N. G. (2012) Development of pyridine-containing macrocyclic copper(II) complexes: potential role in the redox modulation of oxaliplatin toxicity in human breast cells. Free Radic Res. 46(9):1157-66. (10% - experimental work and data analysis)
8. Gonçalves, S., Fernandes, A. S., Oliveira, N. G., Marques, J., Costa, J., Cabral, M. F., Miranda, J., [...], M., Guerreiro, P. S., Castro, M. (2012) Cytotoxic effects of cadmium in mammary epithelial cells: protective role of the macrocycle [15]pyN5. Food Chem Toxicol. 50(6):2180-7. (5% - experimental work and data analysis)

Biotechnologie, Drug Discovery, Pharmakologie, Pharmazie, Biologie