Biotechnologist
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- 1030 Wien, Landstraße
- Weltweit
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- 12.07.2018
Kurzvorstellung
Auszug Referenzen (5)
"Herr Dr. A. [...] war bei uns für die Optimierung einer Error- Prone Methode für einen Milchsäure produzierenden MO mit sehr viel Einsatz tätig."
3/2017 – 8/2017
Tätigkeitsbeschreibung
- I elaborated methods for genetic improvement of B. coagulans strains
- Performed HTS (high through-put screening) and HPLC analysis
- Carried out literature, database mining and NGS data analysis
- Wrote reports and SOPs
- Trained coworkers
Biotechnologie, Naturwissenschaft
"Dr. [...] was a visiting researcher in my group at the Plant Breeding and Genetics Laboratory at the IAEA Laboratories."
10/2016 – 12/2016
Tätigkeitsbeschreibung
- Optimization of pre-NGS amplicon fragmentation protocols
- Writing a scientific paper
- Coworker supervision
Naturwissenschaft
"I was an independent researcher, learning and training myself in different fields for future projects."
1/2015 – 4/2016
Tätigkeitsbeschreibung
I extended my knowledge in several fields including:
Next Generation Sequencing, Induced genome mutagenesis, Genome editing, Exome capture. I trained myself in:
NGS data analysis, at Medical University of Vienna
languages: German, acheived B2, completed B2/C1 course, at VHS Vienna. I also:
Biotechnologie, Angewandte Forschung
"[...] Tramontan hat am AIT ein Jahr lang als Postdoc in einem Projekt über Markerentwicklung bei Kartoffeln mitgearbeitet"
4/2013 – 3/2014
Tätigkeitsbeschreibung
Based on NGS deep sequencing data I established a screening method for large sample populations and MAS (marker assisted selection) for viral and fungal resistance NBS-LRR genes in S. tuberosum. I conducted literature research, carried out all molecular biology steps prior to next generation sequencing, analyzed data, designed allele-specific primers, conducted wet-lab trials, contributed to building genetic map, gave presentations, supervised students.
I also took part in another NGS based project aiming at identifying natural variants of industrial interest of the S. tuberosum GBSS (granule-bound starch synthase) enzyme.
Medizintechnik, Biotechnologie, Interkulturelle Kommunikation, Forschung & Entwicklung, Angewandte Forschung, Biologie, Aus- / Weiterbildung
"Dr. [...] [...] hat seine Doktorarbeit in meiner Gruppe am Max Planck Institut für Pflanzenzüchtungsforschung in Köln durchgeführt."
7/2006 – 4/2011
Tätigkeitsbeschreibung
Between Cologne and Vienna I carried out my PhD research and a bridging postdoc.
I was responsible for the literature research and for laboratory research.
I developed methods for gene mutagenesis crop plants. I gave presentations, wrote scientific publications, participated in international congresses, organized scientific events and supervised students.
Veranstaltungsmanagement, Angewandte Forschung, Kommunikationswissenschaft, Biologie
Qualifikationen
Projekt‐ & Berufserfahrung
3/2017 – 8/2017
Tätigkeitsbeschreibung
- I elaborated methods for genetic improvement of B. coagulans strains
- Performed HTS (high through-put screening) and HPLC analysis
- Carried out literature, database mining and NGS data analysis
- Wrote reports and SOPs
- Trained coworkers
Biotechnologie, Naturwissenschaft
10/2016 – 12/2016
Tätigkeitsbeschreibung
- Optimization of pre-NGS amplicon fragmentation protocols
- Writing a scientific paper
- Coworker supervision
Naturwissenschaft
6/2016 – 8/2016
Tätigkeitsbeschreibung
- RNA-seq of the multipurpose plant Jatropha curcas (physic nut).
- TILLING by Sequencing of J. curcas to identify genes for oil biosynthesis, toxicity and abiotic stress resistance.
- Student training
Biotechnologie, Angewandte Forschung, Naturwissenschaft, Aus- / Weiterbildung
1/2015 – 4/2016
Tätigkeitsbeschreibung
I extended my knowledge in several fields including:
Next Generation Sequencing, Induced genome mutagenesis, Genome editing, Exome capture. I trained myself in:
NGS data analysis, at Medical University of Vienna
languages: German, acheived B2, completed B2/C1 course, at VHS Vienna. I also:
Biotechnologie, Angewandte Forschung
4/2013 – 3/2014
Tätigkeitsbeschreibung
Based on NGS deep sequencing data I established a screening method for large sample populations and MAS (marker assisted selection) for viral and fungal resistance NBS-LRR genes in S. tuberosum. I conducted literature research, carried out all molecular biology steps prior to next generation sequencing, analyzed data, designed allele-specific primers, conducted wet-lab trials, contributed to building genetic map, gave presentations, supervised students.
I also took part in another NGS based project aiming at identifying natural variants of industrial interest of the S. tuberosum GBSS (granule-bound starch synthase) enzyme.
Medizintechnik, Biotechnologie, Interkulturelle Kommunikation, Forschung & Entwicklung, Angewandte Forschung, Biologie, Aus- / Weiterbildung
7/2006 – 4/2011
Tätigkeitsbeschreibung
Between Cologne and Vienna I carried out my PhD research and a bridging postdoc.
I was responsible for the literature research and for laboratory research.
I developed methods for gene mutagenesis crop plants. I gave presentations, wrote scientific publications, participated in international congresses, organized scientific events and supervised students.
Veranstaltungsmanagement, Angewandte Forschung, Kommunikationswissenschaft, Biologie
Zertifikate
Ausbildung
Universität zu Köln
Università di Napoli "Federico II"
Über mich
I would like to work in applied research and possibly bridging between different institutions. For me not only the laboratory research is important, but the real transformation of the results into products.
Weitere Kenntnisse
MOLECULAR BIOLOGY
PCR, RT PCR, Inverse PCR, Multiplex PCR, AS-PCR, Error-prone PCR, qPCR, molecular markers: SNPs, CAPs, SSR, ISSR, RAPDs.
BIOCHEMISTRY
DNA / RNA work Cloning, Agarose Gel Electrophoresis, Southern / Western Blotting, Protein isolation, Protein heterologous expression, Antibody purification, SDS-PAGE, Flow-cytometry, ELISA,, HPLC (Chromeleon), HTS (High Throughput Screening).
MICROBIOLOGY Genetic manipulation of E. coli, A. tumefaciens, Bacillus coagulans. “Heat-shock” transformation, electroporation.
PLANT METHODS
In vitro and in vivo manipulation of T. aestivum, A. thaliana, H. vulgare, S. tuberosum, J. curcas.
NEXT GENERATION SEQUENCING
Illumina, Ion Torrent, workflow management, Data Analysis, NGS Quality Control, Amplicon Sequencing, RNA-seq
GENOMICS
SNP discovery, NBS-profiling, MAS linkage mapping in tetraploid species (first experience), gene annotation, Induced mutagenesis, TILLING by Sequencing, Eco-TILLING
COMPUTER SKILLS
MS Office, Open Office, BioEdit, Sequencer, IGV (Integrated Genome Viewer), Bio-Linux, Clone Manager, CLC Main Workbench, Primer3, CODDLE, QuantityOne (Biorad), Genome Databases, BLAST, MSA (Multi Sequence Alignment), Tetraploid Map, Image J, HTS (High Throughput Screening), Microlab STAR (Hamilton), PROSize, Covaris, Chromeleon, NGS Pipelines, CODDLE, Provean, SIFT, Adobe Acrobat, Adobe Photoshop, SAP.
HARDWARE
All Thermocyclers (PCR), Fragment Analyzer (Applied Biosystems), Hamilton Liquid Handling Workstation, HPLC, Covaris Sonicator, Nanodrop, Qubit, Optical Microscopy.
SOFT SKILLS
GxP
COMMUNICATION
Communication skills gained through professional experience, further training and free time activities
“Talented in presentations”
“Able to simplify complex concepts”
Client relations
TEAM WORKING
Ability and joy to work in a multicultural team / environment
Ability to perform with autonomy, as well as part of a team
ORGANIZATION / MANAGEMENT
Management and coordination of multidisciplinary projects
Ability to establish collaborations with partners in different fields
HOBBIES
Gastronomy (cooking, baking), arts (music, theatre), sports (volleyball, jogging, swimming)
LANGUAGE SKILLS
Italian: Native Speaker
English: Fluent
German: Fluent
Spanish: Fluent
Serbo/Croatian: beginner
Persönliche Daten
- Italienisch (Muttersprache)
- Englisch (Fließend)
- Deutsch (Fließend)
- Spanisch (Fließend)
- Serbisch (Grundkenntnisse)
- Europäische Union
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